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Before agar, microbiologists had experimented with other foodstuffs as microbial media. They turned to substances rich in the starches, proteins, sugars, fats, and minerals that organisms need for growth, testing with broths, bread, potatoes, polenta, egg whites, coagulated blood serums, and gelatine. However, none worked particularly well: all were easily broken down by heat and microbial enzymes, and their surface, once colonized, became mushy and unsuitable for isolating microbes.

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"Numbers have plummeted due to a myriad of different factors including over-abstraction, habitat degradation, pollution, impacts on redds, in-stream barriers, climate change, and pressures associated within the marine environment."